Lab for Single-Cell Nanoengineering (LSCN)

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Lingqian Chang

Times cited at Google Scholar=1600,


h-10 index =39

(Source: Google Scholar            Last Updated: 07/20/2020)

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Book and Chapters


    1. Shulin Li, Lingqian Chang, Justin Tessie, <ElectroporationProtocols>, Springer Nature, Oct 2019, ISBN:978-1-4939-9740-4.

Chapters   (* Correspondence)

  1. L. Chang*, C. Chiang, Y. Jiao, L. Teng, Non-viraltransfection methods for efficient gene delivery: Micro-/nano-technology for electroporation.“The World Scientific Encyclopedia of Nanomedicine and Bioengineering I: Volume 3: Nanotechnology in Gene Delivery”. World Scientific Publisher, Oct2016, 175-218. ISBN: 978-981-4667-65-4

  2. L. Chang*, S. Black, C.Chitrakar, M. Nouri, 3D Nanochannel Array for High-Throughput Cell Manipulationand Electroporation. “Methods in Molecular Biology: ElectroporationProtocols”. Springer Nature, Oct 2019, ISBN: 978-1-4939-9740-4.

  3. L. Chang*, C. Chitrakar, M. Nouri, 3D Nanochannel electroporation for macromolecular nucleotidesdelivery. “Methods in Molecular Biology: Electroporation Protocols”. SpringerNature, Oct 2019, ISBN: 978-1-4939-9740-4.

Peer-reviewed Journal

# Equal Contribution;* Correspondence)


  1. Y.Wang, K. Li, G. Xu, C. Chen, G. Song, Y. Wang, Z. Xu, M. Yu, X. Yu, Y. Fan, B.Ying, L. Chang*, J. Geng*, Low-cost and scalable microwell arrayplatform for colorimetric point-of-care testing of COVID-19. 2020. (Under Review)

  2. Z. Dong,X. Xue, J. Guan, L. Chang*, DNA Nanomachines for Early Detectionof Cancer. 2020. (Under Review)

  3. H.Jia, Y. Wei, T. Weng, C. Virgile, L. Zhang, L. Chang*, Y. Xu, W.Lu, P. Zhang. Engineering biomaterials tomodulate immunity in rheumatoid arthritis for translational purposes. 2020. (Under Review)

  4. Y.Liu, H. Zheng, L. Zhao, S. Liu, K. Yao, D. Li, C. Yiu, S. Gao, R. Avila, C. Pakpong,L. Chang*, Z. Wang*, X. Huang*, Z. Xie*, Z. Yang*, X. Yu*, ElectronicSkin from High-throughput Fabrication of Intrinsically Stretchable Lead ZirconateTitanate Elastomer, Research, 2020.

  5. P.Mukherjee, E.J. Berns, C.A. Patino, E.M. Moully, L. Chang, S.S.P.Nathamgari, J. Kessler, M. Mrksich, H.D. Espinosa, Temporal Sampling ofEnzymes from Live Cells by Localized Electroporation and Quantification ofActivity by SAMDI Mass Spectrometry, Small, 2020, DOI: 10.1002/smll.202000548 (IF: 11.4) (Q1 TOP)

  6. Q.Zhang, X. Wei, Y. Li, Z. Dong, F. Chen, M. Zhong, J. Shen, Z. Liu, L. Chang*, Adjustable and ultrafast light-cure hyaluronic acid hydrogel:promoting biocompatibility and cell growth, Journal of Material Chemistry B, 2020, DOI: 10.1039/c9tb02796c. (IF:5.1) (Q1 Top).

  7. P.Chen, J. Guo, M. Nouri, Q. Tao, Z. Li, Q. Li, L. Du, H. Chen, Z. Dong, L. Chang*, Y. Liu, L. Liao and Y. Hu*, Microfluidic Solution-ProcessedOrganic and Perovskite Nanowires Fabricated for Field-Effect Transistors andPhotodetectors, Journal of Material Chemistry C, 2020, DOI:10.1039/C9TC05628A. (IF: 6.6) (Q1 Top)


  1. Z. Dong, Y. Jiao, Y. Hao, P. Wang, J. Shi, W. An, C. Chichtrakar, S. Black, L.J. Lee, M. Li, Y. Fan, L. Chang*, On-Chip Multiplexed Single-cell Pattern and Controllable Intracellular Delivery. Microsystem and Nanoengineering, 2019. DOI:10.1138/s41378-019-0112-z.   (IF: 5.6) (Q1 Top)   

  2. L. Chang, Y. Wang, F. Ershad, R. Yang, C. Yu, Y. Fan, Wearable Devices for Single Cell Sensing and Transfection. Trends in Biotechnology, 2019, 37, 1175-1188. (IF: 14.3)(Q1 Top)   

  3. Y. Zhu, J. Shen, L.Yin, X. Wei, F. Chen*, M. Zhong, Z. Gu, Y. Xie, W. Jin, Z. Liu, C. Chitrakar, L. Chang, Directly photopatterning of polycaprolactone-derived photocured resin by UV-initiated thiol-ene “click” reaction: Enhanced mechanical property and excellent biocompatibility. Chemical Engineering Journal, 2019, 366, 112-122. (IF:10.6) (Q1 Top)   

  4. F. Chen, Y. Zhu, W.Li, J. Yang, P. Fan, M. Zhong, L. Chang*, Tairong Kuang*, Light-triggeredpH/Thermal Multi-Sensitive Polyelectrolyte/ITO Glass Hybrid Electrode. Applied Surface Science. 2019, 464, 273-279. (IF: 6.1) (Q1 Top)   

  5. J. Ju, X. Peng, K. Huang, L. Li, X. Liu, C. Chitrakar, L. Chang, Z. Gu,T. Kuang, High-performance porous PLLA-based scaffolds for bone tissue engineering: Preparation, characterization, and in vitro and in vivo evaluation. Polymer, 2019, 180, 121-707.

  6. Su, YC, Cockerill, I, Y Wang,YX Qin, L Chang*, Y Zheng*, D Zhu*, Zinc-based biomaterials for regeneration and therapy. Trends in Biotechnology, 2019, 37, 428-441. (IF: 14.3) (Q1 Top)   


  1. X. Wei, J. Shen, Z. Gu, Y. Zhu, F. Chen*, M. Zhong, L. Yin, Y. Xie, Z. Liu, W. Jin, M. Nouri, L.Chang*, Bioinspired pH-Sensitive Surface on Bioinert Substrate, ACS Applied BioMaterials. 2018, 1, 2167-2175.

  2. W. Bai, T. Kuang*, C. Chitrakar, R. Yang, S. Li, D. Zhu, L. Chang*, Patchable Micro/nanodevice Interacting With Skin. Biosensors and Bioelectronics. 2018, 122, 189-204. (IF:10.2) (Q1 Top)   

  3. H. Suo, Z. Wang, G. Dai, J. Fu, J. Yin*, L. Chang*, Polyacrylonitrile Nerve Conduits with Inner Longitudinal Micro-Grooves Enhance Neuron Directional Outgrowth. IEEE Journal of Microelectromechanical Systems. 2018, 27, 457 – 463   

  4. Z. Zhou, F. Chen, T. Kuang, L. Chang, J. Yang, P. Fan, Z. Zhao, M. Zhong, Lignin-derived mesoporous carbon and NiOhybrid nanosphere with exceptional Li-ion battery and pseudo capacitive properties, Electrochimica Acta, 2018, 274, 288 -297. (IF: 6.2) (Q1 Top)   


  1. D. Li, Z. Wan, Y. Jiao, X. Ouyang, L. Chang*, X. Wang*, Bifunctional MoS2 coatedmelamine-formaldehyde Sponges for Efficient Oil-Water Separation and Water-Soluble Dye Removal. Applied Materials Today, 2017, 9, 551 -559. (IF:8.3) (Q1 Top)   

  2. W. Li, T. Kuang, X. Jiang, J. Yang, P. Fan, Z. Zhao, Z. Fei, M. Zhong, L. Chang*, F. Chen*, Photoresponsive Polyelectrolyte/Mesoporous Silica Hybrid Materials with Remote-Controllable Ionic Transportation. Chemical Engineering Journal. 2017.322, 445- 453. (IF: 10.6) (Q1 Top)   

  3. D. Gallego-Perez, D.Pal, S. Ghatak, V. Malkoc, N. Higuita-Castro, S. Gnyawali, L. Chang, W. Liao, J. Shi, M. Sinha, K. Singh, E. Steen, A. Sunyecz, R. Stewart, J. Moore, T. Ziebro, R. Northcutt, M. Homsy, P. Bertani, W. Lu, S. Roy, S. Khanna, C. Rink,V.B. Sundaresan, J. Otero, L.J. Lee*, C. K. Sen*, Topical tissue nano-transfectionmediates non-viral stroma reprogramming and rescue. Nature Nanotechnology. 2017, 12, 974-979. (IF: 31.5) (Q1 Top)   

  4. L. Chang, F. Chen, X. Zhang, T. Kuang, M. Li, J. Hu, J. Shi, L. J. Lee, H. Chen, Y. Li, Synthetic Melanin E-ink. ACS Applied Materials & Interfaces. 2017, 9, 16553-16560. (IF:8.8) (Q1 Top)   

  5. T. Kuang#, L. Chang#*, X. Peng, X. Hu, D. Gallego-perez, Molecular beacon nano-sensorsprobing living cancer cells. Trends in Biotechnology. 2017, 35, 347-359. (IF:14.3)(Q1 Top)   

  6. F. Chen, Z. Zhou, L. Chang, T. Kuang, Z. Zhao, P. Fan, J. Yang, M. Zhong, Synthesis and Characterization of Lignosulfonate-Derived Hierarchical Porous Graphitic Carbons for Electrochemical Performances. Microporous and Mesoporous Materials. 2017, 247, 184-189.

  7. T.Kuang, F. Chen, L. Chang, X.Peng, X. Gong, Facile Preparation of Open-cellular Porous Poly (L-lactic acid) Scaffold by Supercritical Carbon Dioxide Foaming for Tissue EngineeringApplications. Chemical Engineering Journal. 2017, 307, 1017-1025. (IF: 10.6) (Q1 Top)   


  1. L. Chang, L. Li, J. Shi, Y. Sheng, W. Lu, D. Gallego-Perez, L. J. Lee. Micro-/nano-scale electroporation. Lab Chip. 2016, 16, 4047-4062. (IF: 6.7) (Q1 Top)   

  2. L. Chang, D. Gallego-perez, C. Chiang, P. Bertani, T. Kuang, F. Chen, Y. Sheng, Z. Chen, J.Shi, H. Yang, V. Malkoc, W. Lu, L. J. Lee, Controllable Large-scale Transfectionof Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform. Small. 2016, 12, 5971-5980. (Cover Story) (IF: 11.4) (Q1 Top)   

  3. D. Gallego-Perez#, L. Chang#, J. Shi, J. Ma, S.H. Kim, X. Zhao, V. Malkoc, X. Wang, M. Minata, K. J. Kwak, Y. Wu, G. Lafyatis, W. Lu, D. J. Hansford, I. Nakano, L. J. Lee, On-chipclonal analysis of glioma stem cells motility and therapy resistance. Nano Letters. 2016, 16, 5326-5332. (IF: 11.2) (Q1 Top)   

  4. L. Chang, P. Bertani, D. Gallego-Perez, Z. Yang, C. L. Chiang, V. Malkoc, L. J. Lee, W. Lu. 3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control. Nanoscale. 2016, 8, 243-252. (ESI Highly cited paper) (Cover Story) (Annual HOT Article) (IF: 6.8) (Q1 TOP)

  5. L. Chang, J. Hu, F. Chen, J. Shi, Z. Yang, Y. Li, L. J. Lee, Nanoscale bio-platforms for living cell interrogation: current status and future perspectives. Nanoscale. 2016, 8, 3138-3206. (IF: 6.8) (Q1 Top)   

  6. T. Kuang#, L. Chang#, F. Chen, Y. Sheng, D. Fu, X. Peng, Facile preparation of light weight high-strength biodegradable polymer nanocomposite foams for electro magnetic interference shielding. Carbon. 2016, 105, 305-313. (ESI Highly cited paper) (IF: 8.8) (Q1 Top)   

  7. Y. Sheng, L. Chang*, J. Hu, T. Kuang, PEG/heparin decorated lipid-polymer hybrid nanoparticles for long-circulating drug delivery. RSC Advances. 2016. 6, 23279-23287.

  8. T.Kuang, L. Chang, D. Fu, J.Yang, M. Zhong, F. Chen, X. Peng, Improved Crystallizability and Process abilityof Ultrahigh Molecular Weight Polyethylene Modified by Poly (amido amine) Dendrimers, Polymer Engineering & Science. 2016, 57, 153-160.

  9. T. Kuang, F. Chen, D. Fu, L. Chang, X. Peng, L. J. Lee, Enhanced Strength and Foamability of High-Density Polyethylene Prepared by Pressure-Induced Flow and Low-Temperature Crosslinking. RSC Advance. 2016, 6, 34422-24427.

  10. K. Gao, X. Huang, C. L. Chiang, X. Wang, L. Chang, P. Boukany, G. Marcucci, R. Lee, L. J. Lee, Induced Apoptosis Investigation in Wild–Type and FLT3-ITD Acute Myeloid Leukemia Cells by Nanochannel Electroporation and Single Cell qRT-PCR, Molecular Therapy, 2016, 24, 956-964. (IF: 8.9) (Q1 Top)   

  11. Z. Chen, A. Zhang, Z. Yang, X. Wang, L. Chang, Z. Chen, L. J. Lee, Application of DODMA and Derivatives in Cationic Nanocarriers for Gene Delivery, Current Organic Chemistry, 2016, 20,1813-1819.

  12. T. Kuang, D. Fu, L. Chang, Z.Yang, J. Yang, P. Fan, M. Zhong, F. Chen, X. Peng, Enhanced Photocatalysis of Yittium-doped TiO2/D-PVA Composites: Degradation of Methyl Orange (MO) and PVCFilm. Science of Advanced Materials. 2016, 8, 1286-1292.

  13. M. Li, B. Li, X. Zhang, Y. Song, L. Chang, Y. Chen, Investigation of the phase fluctuation effect on the BER performance of DPSK space downlink optical communication system onfluctuation channel, Optics Communications, 2016, 366,248-252.


  1. L. Chang, M. Howdyshell, W. Liao, C. Chiang, D. Gallego-Perez, Z. Yang, J. Byrd, L. Wu, N. Muthusamy, L.J. Lee. R. Sooryakumar. Magnetic tweezer based 3D micro-electroporation system for high throughput transfection in living cells. Small. 2015, 11, 1818-1828. (ESI Highly cited paper) (IF: 11.4)(Q1 Top)   

  2. L. Chang, D. Gallego-Perez, X. Zhao, P. Bertani, Z. Yang, C. L. Chiang,   V. Malkoc, C. K. Sen, L. Odonnell, J. Yu, W. Lu, L.J. Lee. Dielectrophoresis-assisted 3D Nanoelectroporation for Non-viral Cell Transfection in Adoptive Immunotherapy. Lab Chip. 2015, 15, 3147 – 3153. (Annual HOT Article) (IF: 6.8) (Q1 Top)   

  3. Z. Yang#, L. Chang#, C. Chiang, L. J. Lee. Micro-/nano-electroporation for active gene delivery. Current Pharmaceutical Design, 2015, 21, 6081-6088.

  4. P. Bertani#, L. Chang#, D. Gallego-Perez, C. Chiang, R. Muthusamy, L. J. Lee, W. Lu, Boschetching for the creation of a 3D nano-electroporation system. Journal of Vacuum Science & Technology B. 2015. 33, 06F903.

  5. V. Malkoc, L. Chang*, Applications of Electrospun Nanofibers in Neural Tissue Engineering. European Journal of   BioMedical Research. 2015, 3, 25-29.

  6. F. Chen, X. Jiang, T. Kuang, L. Chang, D. Fu, J. Yang, P. Fan, M. Zhong. Polyelectrolyte/Mesoporous Silica Hybrid Materials for High Performance Multiple-Detection of pH Value and Temperature. Polymer Chemistry. 2015, 6, 3529 -3536. (IF:5.3)(Q1 Top)   

  7. R. Sun, L. Chang, L. Li.Manufacturing polymeric micro lens and self-organised micro lens arrays by using microfluidic dispensers. Journal of Micromechanics and Microengineering. 2015, 25, 115012 -115222.

  8. F. Chen, X. Jiang, T. Kuang, L. Chang, D. Fu, Z. Yang, J. Yang, P. Fan, Z. Fei, M. Zhong, Effect of NanoporousStructure and Polymer Brushes on the Ionic Conductivity of Poly (methacrylicacid)/Anode Aluminum Oxide Hybrid Membrane, RSC Advances, 2015, 5, 70204-70210.

  9. Z. Chen, Y. Yang, Z. Yang, J. Hu, T. Xu, L. Chang, L.J. Lee, C. Sheng, Z. Chen, Preparation and characterization of vacuum insulation panels with super-stratified glass fiber core material. Energy. 2015, 93, 945-954. (IF: 6.0) (Q1 Top)   

  10. T. Kuang, D. Fu, L. Chang, Z. Yang, Z. Chen, L. Jin, F. Chen, X. Peng. Recent Progress in Dendrimer-based Gene Delivery Systems. Current Organic Chemistry. 2015. 20, 1820-1826.

  11. M. Li, B. Li, Y. Song, X. Zhang, L. Chang, J. Liu, Investigation of Costas loop synchronization effect on BER performance of space uplink optical communication system with BPSK scheme, IEEE Photonics Journal, 2015, 7, 7902309.

  12. M. Li, W. Jiao, X. Liu, Y. Song, L.Chang, A method for peak seeking of BOTDR based on the incomplete brillouin spectrum, IEEE Photonics Journal, 2015,7, 7102110.

  13. M. Li, W. Jiao, Y. Song, X. Zhang, L. Chang, Self-adaptive high anti-radiation EDFA for space optical communication systems. Journal of Lightwave Technology, 2015, 33, 4513 - 4516.

Before 2015

  1. L. Chang, C. Liu, Y. He, H. Xiao, X. Cai, Small-volume Solution Current-Time Behavior Study for Application in Reverse Iontophoresis based Non-invasive Blood Glucose Monitoring, Science China Chemistry, 2011, 54,223-230. (IF: 6.3)

  2. K. Gao, L. Li, L. He, K. Hinkle, Y. Wu, J. Ma, L. Chang, X. Zhao, D. Gallego-Perez, S.Eckardt, J. Mclaughlin, B. Liu, D.F. Farson, L.J. Lee. Design of a Microchannel-Nanochannel-Microchannel Array Based Nanoelectroporation Systemfor Precise Gene Transfection, Small, 2014, 10, 1015-1023. (IF: 11.4) (Top)

  3. P. Xie, P. He, Y.-C. Yen, K.J. Kwak, D. Gallego-Perez, L. Chang, W.-C. Liao, A. Yi, L.J. Lee. Rapid hot embossing of polymer microstructures using carbide-bonded graphene coating on silicon stampers. Surface & Coatings Technology, 2014, 258, 174-180.

  4. H. Xiao,L. Chang, Q. Yang, Y. He, X. Cai, Development and experiment of a Glucose Monitor using noninvasive transdermalextraction, Chinese Journal of Scientific Instrument, 2010, 31, 2796-2802.

  5. Y. He, H. Xiao, L. Chang, C. Liu, X. Cai, Design of a Low-power Non-invasive Glucose Monitor and Test of Its Performance, Chinese Journal Sensors and Actuators, 2010, 23, 903-907.

  6. J. Wang, M. Su, J. Qi, L. Chang, Sensitivity and complex impedance of nanometer zirconia thick film humidity sensors, Sensorsand Actuators B: Chemical, 2009, 139, 418-424.   (IF: 6.4)(Q1 Top)   



  1. L. Chang, R. Lyle Hood, Forhad Akhter. Microneedle array electroporation system for cell transfection. Filing Date: 05/2018. (Provisional)

  2. L. Chang, Y. Hao, I. Muhbub, D. Zhu, H. Chang. Nano-devices for skin and mucosal macromolecule delivery and detection. Filing Date: 02/2018. Patent NO. 62628617 (Provisional)

  3. D. Gallego-Perez, C. Sen, N. Higuita-Castro, L. Chang. Interpenetrating Nanochannels for Deep-Topical Tissue Nanoelectroinjection (DTN). Filing Date: 06/2016. Patent NO: US20190329014A1.


  1. 常凌乾,王杨,耿佳,应斌武,樊瑜波,李为民,新型冠状病毒环介导等温扩增检测芯片及制备和使用方法,申请号:2020010537454.8

  2. 常凌乾,王杨,耿佳,应斌武,新型冠状病毒环介导等温扩增检测及试剂盒,申请号:202010541335.X

  3. 常凌乾,王杨,耿佳,应斌武,新型冠状病毒环介导等温扩增检测及试剂盒—1申请号:202010537448.1

  4. 常凌乾,王杨,耿佳,应斌武,新型冠状病毒环介导等温扩增检测及试剂盒—2申请号:202010537448.2

  5. 常凌乾,郝永存,常洪龙,一种向细胞递送目标分子的生物芯片及其制备方法和应用,发明专利,公开日期:11/2019公开号:110452807A

  6. 常凌乾,陈枫,郝永存, 一种检测微传感器的结构及其检测方法,发明专利,公开日期:11/2019,公开号:110455887A

  7. 李乙文,常凌乾,陈枫,张小康,电子墨水显示材料及其制备办法,发明专利,公开日期:07/2017,公开号:106947324B

  8. 肖宏辉;蔡新霞;刘军涛;常凌乾,一种基于手机网络的家用远程无线医疗监护系统, 发明专利, 公开日期: 11/2012,公开号:102801751A

  9. 肖宏辉;蔡新霞;常凌乾;刘春秀, 一种手持式多参数现场快速生化检测仪, 发明专利, 公开日期: 07/2012,公开号:102539774A